The pH dependence of dealkylation in soman-inhibited cholinesterases and their mutants: Further evidence for a push-pull mechanism

Ashima Saxena, Carol Viragh, D. Scott Frazier, Ildiko M. Kovach, Donald M. Maxwell, Oksana Lockridge, B. P. Doctor

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Bimolecular rate constants for the inactivation of recombinant (r) human (Hu) butyrylcholinesterase (BChE) with P(S)C(S)- and P(S)C(R)-2-(3,3- dimethylbutyl) methylphosphonofluoridate (soman) are (92 ± 7) x 106 M-1 min-1 and (13.7 ± 0.8) x 106 M1-1 min-1 at pH 7.4, μ = 0.1 M and 25 °C. Mutations of E197(199) to D or Q and W82(84) to A result in reductions in the rate constants for reactivation with P(S)C(S)-soman 4.3-, 11.8-, and 263-fold and with P(S)C(R)-soman by 6.5-, 47.3-, and 685-fold, respectively. The pH dependence of dealkylation (aging) in r mouse (Mo) acetylcholinesterase (AChE) and rHu BChE and their mutants inactivated with P(S)C(S)- and P(S)C(R)-soman was compared. Best-fit parameters for the asymmetric bell curves for the adducts of wild-type Mo AChE are pK1 = pK2 = 4.0-4.9 and pK3 = 5.2-6.6. These pKs are consistent with the involvement of two carboxylic acids, possibly E202(199) and either E334(327) or E450(443), and H447(440)H+ in the dealkylation of AChE. E202Q MoAChE inactivated with the soman diastereomers yielded pK3 = 5.5-5.8. Nearly symmetric pH curves for soman-inhibited wild-type and E197D Hu BChE gave pK2 = 3.7-4.6 and pK3 = 7.3-8.0, but much lower, pK3 ~5, for the corresponding adduct of the E197Q mutant. Dealkylation in soman-inhibited BChE is consistent with the participation of one carboxylic acid side chain and H438(440)H+ Maximal rate constants for dealkylation (k(max)) are 1-6 min-1 for AChE and 2 min-1 for BChE at 25 °C. The W82 to A mutation in BChE results in the largest reduction, 2500-6000-fold, in the rate constant for dealkylation. The reduction in the rate constants for dealkylation in the E197 mutants is highly pH dependent. The solvent isotope effects at the pH maxima are 1.3- 1.4, indicating unlikely preprotonation or proton in 'flight' at the enzymic transition states. The new results support the push-pull mechanism of dealkylation in soman-inhibited cholinesterases proposed previously.

Original languageEnglish (US)
Pages (from-to)15086-15096
Number of pages11
JournalBiochemistry
Volume37
Issue number43
DOIs
StatePublished - Oct 27 1998

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'The pH dependence of dealkylation in soman-inhibited cholinesterases and their mutants: Further evidence for a push-pull mechanism'. Together they form a unique fingerprint.

Cite this