Abstract
The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.
Original language | English (US) |
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Pages (from-to) | 63-67 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 65 |
Issue number | 1 |
DOIs | |
State | Published - Jul 8 1975 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology