The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

M. G. Brattain; C. M. Jones; J. M. Pittman; T. G. Pretlow

doi:10.1016/S0006-291X(75)80061-1

The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

M. G. Brattain, C. M. Jones, J. M. Pittman, T. G. Pretlow

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Abstract

The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.

Original language	English (US)
Pages (from-to)	63-67
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	65
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(75)80061-1
State	Published - Jul 8 1975
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(75)80061-1

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title = "The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A",

abstract = "The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.",

author = "Brattain, {M. G.} and Jones, {C. M.} and Pittman, {J. M.} and Pretlow, {T. G.}",

note = "Funding Information: iSupported by grants from the National Institutes of Health (CA-13148, CA-16764, CA-15089, CA-16430, and DE-2670) and the American Cancer Society (DT-43A). 2Currently Intern in Department of Internal Medicine, University of Alabama Medical School. 3Reeipient of United States Public Health Service Research Career Development Award K4-CA-70584-03.",

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T1 - The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

AU - Brattain, M. G.

AU - Jones, C. M.

AU - Pittman, J. M.

AU - Pretlow, T. G.

N1 - Funding Information: iSupported by grants from the National Institutes of Health (CA-13148, CA-16764, CA-15089, CA-16430, and DE-2670) and the American Cancer Society (DT-43A). 2Currently Intern in Department of Internal Medicine, University of Alabama Medical School. 3Reeipient of United States Public Health Service Research Career Development Award K4-CA-70584-03.

PY - 1975/7/8

Y1 - 1975/7/8

N2 - The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.

AB - The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.

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The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

Abstract

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