The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 8 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology