@article{b2f017c9ed874a3b80760d7fec91520c,
title = "The purification of horseradish peroxidase by affinity chromatography on Sepharose-bound concanavalin A",
abstract = "This report describes the use of affinity chromatography on Sepharose-bound concanavalin A for the purification of horseradish peroxidase. Samples of horseradish peroxidase with A403:A280 ratios ranging from 0.62 to 2.45 were purified to A403:A280 ratios ranging from approximately 2.8 to 3.1 with the recovery of 73% or more of the enzymatic activity. Characterization of the purified horseradish peroxidase showed an increased enzymatic activity with respect to both absorbance at 403 nm and protein content.",
author = "Brattain, {Michael G.} and Marks, {Michael E.} and Pretlow, {Thomas G.}",
note = "Funding Information: I To be presented at the meeting of the American Society for Experimental Pathology, Anaheim, California, April, 1976. * This investigation was supported by Public Health Service Grants CA-15089 from the National Cancer Institute, CA-16764 from the National Cancer Institute through the National Large Bowel Cancer Project, CA-16430 from the National Cancer Institute through the National Prostatic Cancer Project, DE-2670 from the National Institute of Dental Research, by American Cancer Society Grant PDT-9B. and by Grant IN66-0. an institutional grant from the American Cancer Society administered by the University of Alabama in Birmingham Cancer Center. 3 Recipient of NIH Research Career Development Award (K4-CA-70584-04) to whom requests for reprints should be addressed.",
year = "1976",
month = may,
day = "7",
doi = "10.1016/0003-2697(76)90540-6",
language = "English (US)",
volume = "72",
pages = "346--352",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1-2",
}