The rDNA internal transcribed spacer region as a taxonomic marker for nematodes

T. O. Powers; T. C. Todd; A. M. Burnell; P. C.B. Murray; C. C. Fleming; A. L. Szalanski; B. A. Adams; T. S. Harris

The rDNA internal transcribed spacer region as a taxonomic marker for nematodes

T. O. Powers, T. C. Todd, A. M. Burnell, P. C.B. Murray, C. C. Fleming, A. L. Szalanski, B. A. Adams, T. S. Harris

Plant Pathology

Research output: Contribution to journal › Article › peer-review

177 Scopus citations

Abstract

The ITS region from a wide taxonomic range of nematodes, including secernentean and adcnophorcan taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITSI is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics.

Original language	English (US)
Pages (from-to)	441-450
Number of pages	10
Journal	Journal of Nematology
Volume	29
Issue number	4
State	Published - Dec 1997

Keywords

Diagnosis
Genetics
ITS
Internal transcribed spacer region
Molecular ecology
Nematode
Systematics
Taxonomy

ASJC Scopus subject areas

Agronomy and Crop Science

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title = "The rDNA internal transcribed spacer region as a taxonomic marker for nematodes",

abstract = "The ITS region from a wide taxonomic range of nematodes, including secernentean and adcnophorcan taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITSI is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics.",

keywords = "Diagnosis, Genetics, ITS, Internal transcribed spacer region, Molecular ecology, Nematode, Systematics, Taxonomy",

author = "Powers, {T. O.} and Todd, {T. C.} and Burnell, {A. M.} and Murray, {P. C.B.} and Fleming, {C. C.} and Szalanski, {A. L.} and Adams, {B. A.} and Harris, {T. S.}",

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AU - Powers, T. O.

AU - Todd, T. C.

AU - Burnell, A. M.

AU - Murray, P. C.B.

AU - Fleming, C. C.

AU - Szalanski, A. L.

AU - Adams, B. A.

AU - Harris, T. S.

PY - 1997/12

Y1 - 1997/12

N2 - The ITS region from a wide taxonomic range of nematodes, including secernentean and adcnophorcan taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITSI is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics.

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KW - Diagnosis

KW - Genetics

KW - ITS

KW - Internal transcribed spacer region

KW - Molecular ecology

KW - Nematode

KW - Systematics

KW - Taxonomy

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