Abstract
The ITS region from a wide taxonomic range of nematodes, including secernentean and adcnophorcan taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITSI is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics.
Original language | English (US) |
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Pages (from-to) | 441-450 |
Number of pages | 10 |
Journal | Journal of Nematology |
Volume | 29 |
Issue number | 4 |
State | Published - Dec 1997 |
Keywords
- Diagnosis
- Genetics
- ITS
- Internal transcribed spacer region
- Molecular ecology
- Nematode
- Systematics
- Taxonomy
ASJC Scopus subject areas
- Agronomy and Crop Science