TY - JOUR
T1 - The relationship between nutrition, gut dysbiosis, and pediatric sickle cell pain outcomes
T2 - A pilot study
AU - Dike, Chinenye R.
AU - Hanson, Corrine
AU - Davies, H. Dele
AU - Obaro, Stephen
AU - Yu, Fang
AU - Harper, James
AU - Grace, Helen
AU - Lebensburger, Jeffrey
AU - Raulji, Chittalsinh
AU - Ma, Jihyun
AU - Mannon, Peter
N1 - Funding Information:
We would like to thank Dr. Yao for help with DNA extraction, and Dr. Peng and the UNMC bioinformatics core for help with initial analysis of the 16S rRNA gene sequencing results, University of Nebraska DNA Sequencing Core for the 16S rRNA gene sequencing, Matt Anderson MPH for help with analysis of quality-of-life questionnaires, Research coordinators (Evan Roberts MBA, Kim Abraham RN, Greta Carlson BS) from the Child Health Research Institute, Nicholas Reynolds MS (Data Management support from the Child Health Research Institute), and the microbiome core at University of Nebraska Medical Center. We would also like to thank all the children who participated in this study and their families. Funding was provided by the Child Health Research Institute at University of Nebraska Medical Center (Chinenye R. Dike, PI), Clinical Research Center at Nebraska Medicine (Chinenye R. Dike, PI), and University of Nebraska Medical Center, Diversity Grant (Chinenye R. Dike, PI).
Funding Information:
We would like to thank Dr. Yao for help with DNA extraction, and Dr. Peng and the UNMC bioinformatics core for help with initial analysis of the 16S rRNA gene sequencing results, University of Nebraska DNA Sequencing Core for the 16S rRNA gene sequencing, Matt Anderson MPH for help with analysis of quality‐of‐life questionnaires, Research coordinators (Evan Roberts MBA, Kim Abraham RN, Greta Carlson BS) from the Child Health Research Institute, Nicholas Reynolds MS (Data Management support from the Child Health Research Institute), and the microbiome core at University of Nebraska Medical Center. We would also like to thank all the children who participated in this study and their families. Funding was provided by the Child Health Research Institute at University of Nebraska Medical Center (Chinenye R. Dike, PI), Clinical Research Center at Nebraska Medicine (Chinenye R. Dike, PI), and University of Nebraska Medical Center, Diversity Grant (Chinenye R. Dike, PI).
Publisher Copyright:
© 2023 The Authors. Pediatric Blood & Cancer published by Wiley Periodicals LLC.
PY - 2023/7
Y1 - 2023/7
N2 - Background: Nutritional deficiencies are prevalent in sickle cell disease (SCD) and may be associated with worse pain outcomes. Gut dysbiosis has been reported in patients with SCD and may contribute to both nutritional deficiencies and pain. Objectives: We tested the association of nutrition, fat-soluble vitamin (FSV) deficiency, and gut microbiome composition on clinical outcomes in SCD. Second, we measured the association between diet and exocrine pancreatic function on FSV levels. Methods: Using case control design, we enrolled children with SCD (n = 24) and matched healthy controls (HC; n = 17, age, sex, race/ethnicity). Descriptive statistics summarized demographic and clinical data. Wilcoxson-rank tests compared FSV levels between cohorts. Regression modeling tested the association between FSV levels and SCD status. Welch's t-test with Satterthwaite adjustment evaluated associations between microbiota profiles, SCD status, and pain outcomes. Results: Vitamin A and D levels were significantly decreased in participants with HbSS as compared to HC (vitamin A, p = <.0001, vitamin D, p =.014) independent of nutritional status. FSV correlated with dietary intake in SCD and HC cohorts. Gut microbial diversity was reduced in hemoglobin SS (HbSS) compared to hemoglobin SC (HbSC) and HC, p =.037 and.059, respectively. The phyla Erysipelotrichaceae and Betaproteobacteria were higher in SCD children reporting the highest quality-of-life (QoL) scores (p =.008 and.049, respectively), while Clostridia were higher in those with lower QoL scores (p =.03). Conclusion: FSV deficiencies and gut dysbiosis are prevalent in children with SCA. Gut microbial composition is significantly different in children with SCD with low QoL scores.
AB - Background: Nutritional deficiencies are prevalent in sickle cell disease (SCD) and may be associated with worse pain outcomes. Gut dysbiosis has been reported in patients with SCD and may contribute to both nutritional deficiencies and pain. Objectives: We tested the association of nutrition, fat-soluble vitamin (FSV) deficiency, and gut microbiome composition on clinical outcomes in SCD. Second, we measured the association between diet and exocrine pancreatic function on FSV levels. Methods: Using case control design, we enrolled children with SCD (n = 24) and matched healthy controls (HC; n = 17, age, sex, race/ethnicity). Descriptive statistics summarized demographic and clinical data. Wilcoxson-rank tests compared FSV levels between cohorts. Regression modeling tested the association between FSV levels and SCD status. Welch's t-test with Satterthwaite adjustment evaluated associations between microbiota profiles, SCD status, and pain outcomes. Results: Vitamin A and D levels were significantly decreased in participants with HbSS as compared to HC (vitamin A, p = <.0001, vitamin D, p =.014) independent of nutritional status. FSV correlated with dietary intake in SCD and HC cohorts. Gut microbial diversity was reduced in hemoglobin SS (HbSS) compared to hemoglobin SC (HbSC) and HC, p =.037 and.059, respectively. The phyla Erysipelotrichaceae and Betaproteobacteria were higher in SCD children reporting the highest quality-of-life (QoL) scores (p =.008 and.049, respectively), while Clostridia were higher in those with lower QoL scores (p =.03). Conclusion: FSV deficiencies and gut dysbiosis are prevalent in children with SCA. Gut microbial composition is significantly different in children with SCD with low QoL scores.
KW - children
KW - fat-soluble vitamins
KW - gut dysbiosis
KW - nutrition
KW - sickle cell disease
UR - http://www.scopus.com/inward/record.url?scp=85156096213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85156096213&partnerID=8YFLogxK
U2 - 10.1002/pbc.30397
DO - 10.1002/pbc.30397
M3 - Article
C2 - 37101362
AN - SCOPUS:85156096213
SN - 1545-5009
VL - 70
JO - Pediatric Blood and Cancer
JF - Pediatric Blood and Cancer
IS - 7
M1 - e30397
ER -