The relative importance of exogenous and substrate-derived nitrogen for microbial growth during leaf decomposition

B. M. Cheever, J. R. Webster, E. E. Bilger, S. A. Thomas

Research output: Contribution to journalArticlepeer-review

Abstract

Heterotrophic microbes colonizing detritus obtain nitrogen (N) for growth by assimilating N from their substrate or immobilizing exogenous inorganic N. Microbial use of these two pools has different implications for N cycling and organic matter decomposition in the face of the global increase in biologically available N. We used sugar maple leaves labeled with 15N to differentiate between microbial N that had been assimilated from the leaf substrate (enriched with 15N) or immobilized from the water (natural abundance 15N: 14N) in five Appalachian streams ranging in ambient NO3-N concentrations from about 5 to 900 μg NO 3-N/L. Ambient NO3- concentration increased sugar maple decomposition rate but did not influence the proportion of microbial N derived from substrate or exogenous pools. Instead, these proportions were strongly influenced by the percentage of detrital ash-free dry mass (AFDM) remaining. Substrate-derived N made up a large proportion of the microbial N after the first 24 h in all streams. Detrital and microbial isotopic 15N signatures approached that of the water as decomposition progressed in all streams, suggesting that exogenous N may be the predominant source of N for meeting microbial requirements even when exogenous N concentrations are low. Our results support predictions of more rapid decomposition of organic matter in response to increased N availability and highlight the tight coupling of processes driving microbial N cycling and organic matter decomposition.

Original languageEnglish (US)
Pages (from-to)1614-1625
Number of pages12
JournalEcology
Volume94
Issue number7
DOIs
StatePublished - Jul 2013

Keywords

  • N
  • Assimilation
  • Chloroform fumigation
  • Heterotrophic microbes
  • Immobilization
  • Nitrogen availability
  • Nitrogen cycling
  • Organic matter decomposition
  • Streams

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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