TY - JOUR
T1 - The role of RFamide-related peptide 3 (RFRP3) in regulation of the neuroendocrine reproductive and growth axes of the boar
AU - Thorson, Jennifer F.
AU - Desaulniers, Amy T.
AU - Lee, Chanho
AU - White, Brett R.
AU - Ford, J. Joe
AU - Lents, Clay A.
N1 - Funding Information:
This project was supported by the Agriculture and Food Research Initiative Competitive Grant No. 2011-67015-30059 from the USDA National Institute of Food and Agriculture. The authors thank Michelle McManus, Ginger Mills, Jenell Wood, and USMARC Swine Operations for technical assistance; and to Linda Parnell for assistance with preparation of the manuscript.
Publisher Copyright:
© 2015 .
PY - 2015/8/1
Y1 - 2015/8/1
N2 - RFamide-related peptide 3 (RFRP3) has been implicated in regulating reproduction and growth. This regulation appears to be dependent upon sex, species, physiological status, and developmental stage. The objective of the present study was to evaluate the effects of RFRP3 on circulating concentrations of luteinizing hormone (LH) and growth hormone (GH) in mature boars. The hypothesis was RFRP3 would reduce circulating concentrations of LH and increase concentrations of GH. Meishan boars (716.6. ±. 2.8 days of age 125.0. ±. 12.4. kg BW) were randomly assigned to treatment: saline (. n=. 4) or RFRP3 (8.5. mg; n=. 5). Plasma was collected at 15-min intervals during 3 periods: pre-treatment, treatment, and post-treatment. During the treatment period, saline or RFRP3 were administered at 15-min intervals. Treatment was administered as a loading dose of 5. mg RFRP3, followed by seven repeated injections of 0.5. mg RFRP3. Pulsatile secretion of LH and GH were not affected by saline treatment. Mean concentrations of LH in RFRP3-treated boars were greater (. P<. 0.01) in the pre-treatment period than in the treatment and post-treatment periods; however, the individual response to RFRP3 challenge was varied. RFRP3 suppressed (. P<. 0.05) mean concentrations of GH during the treatment period. It is concluded that RFRP3 can act to suppress LH secretion in some boars, but the minimal and varied response between animals does not strongly support the idea that RFRP3 is a potent hypohysiotropic hormone in the pig. Results indicate that RFRP3 may function in regulating the growth axis of swine.
AB - RFamide-related peptide 3 (RFRP3) has been implicated in regulating reproduction and growth. This regulation appears to be dependent upon sex, species, physiological status, and developmental stage. The objective of the present study was to evaluate the effects of RFRP3 on circulating concentrations of luteinizing hormone (LH) and growth hormone (GH) in mature boars. The hypothesis was RFRP3 would reduce circulating concentrations of LH and increase concentrations of GH. Meishan boars (716.6. ±. 2.8 days of age 125.0. ±. 12.4. kg BW) were randomly assigned to treatment: saline (. n=. 4) or RFRP3 (8.5. mg; n=. 5). Plasma was collected at 15-min intervals during 3 periods: pre-treatment, treatment, and post-treatment. During the treatment period, saline or RFRP3 were administered at 15-min intervals. Treatment was administered as a loading dose of 5. mg RFRP3, followed by seven repeated injections of 0.5. mg RFRP3. Pulsatile secretion of LH and GH were not affected by saline treatment. Mean concentrations of LH in RFRP3-treated boars were greater (. P<. 0.01) in the pre-treatment period than in the treatment and post-treatment periods; however, the individual response to RFRP3 challenge was varied. RFRP3 suppressed (. P<. 0.05) mean concentrations of GH during the treatment period. It is concluded that RFRP3 can act to suppress LH secretion in some boars, but the minimal and varied response between animals does not strongly support the idea that RFRP3 is a potent hypohysiotropic hormone in the pig. Results indicate that RFRP3 may function in regulating the growth axis of swine.
KW - Boar, Growth hormone
KW - Luteinizing hormone
KW - Neuroendocrinology
KW - RFamide-related peptide 3
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U2 - 10.1016/j.anireprosci.2015.05.013
DO - 10.1016/j.anireprosci.2015.05.013
M3 - Article
C2 - 26051609
AN - SCOPUS:84937520908
SN - 0378-4320
VL - 159
SP - 60
EP - 65
JO - Animal Reproduction Science
JF - Animal Reproduction Science
ER -