The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cells

Gautam K. Malhotra, Xiangshan Zhao, Emily Edwards, Janel L. Kopp, Mayumi Naramura, Maike Sander, Hamid Band, Vimla Band

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Background: Identification and characterization of molecular controls that regulate mammary stem and progenitor cell homeostasis are critical to our understanding of normal mammary gland development and its pathology. Results: We demonstrate that conditional knockout of Sox9 in the mouse mammary gland results in impaired postnatal development. In short-term lineage tracing in the postnatal mouse mammary gland using Sox9-CreER driven reporters, Sox9 marked primarily the luminal progenitors and bipotent stem/progenitor cells within the basal mammary epithelial compartment. In contrast, long-term lineage tracing studies demonstrate that Sox9+ precursors gave rise to both luminal and myoepithelial cell lineages. Finally, fate mapping of Sox9 deleted cells demonstrates that Sox9 is essential for luminal, but not myoepithelial, lineage commitment and proliferation. Conclusions: These studies identify Sox9 as a key regulator of mammary gland development and stem/progenitor maintenance.

Original languageEnglish (US)
Article number47
JournalBMC Developmental Biology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • Cre-lox
  • Knockout
  • Luminal progenitor cells
  • Mammary gland development
  • SOX9
  • Stem cells

ASJC Scopus subject areas

  • Developmental Biology

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