The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity

Cesar Augusto Medina; Paola Andrea Reyes; Cesar Augusto Trujillo; Juan Luis Gonzalez; David Alejandro Bejarano; Nathaly Andrea Montenegro; Jonathan M. Jacobs; Anna Joe; Silvia Restrepo; James R. Alfano; Adriana Bernal

doi:10.1111/mpp.12545

The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity

Cesar Augusto Medina, Paola Andrea Reyes, Cesar Augusto Trujillo, Juan Luis Gonzalez, David Alejandro Bejarano, Nathaly Andrea Montenegro, Jonathan M. Jacobs, Anna Joe, Silvia Restrepo, James R. Alfano, Adriana Bernal

Plant Pathology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) using heterologous systems. AvrBs2, XopR and XopAO1 are capable of suppressing PTI. ETI suppression activity was only detected for XopE4 and XopAO1. These results demonstrate the overall importance and diversity in functions of major virulence effectors AvrBs2 and XopAO1 in Xam during cassava infection.

Original language	English (US)
Pages (from-to)	593-606
Number of pages	14
Journal	Molecular Plant Pathology
Volume	19
Issue number	3
DOIs	https://doi.org/10.1111/mpp.12545
State	Published - Mar 2018

Keywords

ETI
PTI
type III effectors
virulence, Xanthomonas axonopodis pv. manihotis

ASJC Scopus subject areas

Molecular Biology
Agronomy and Crop Science
Soil Science
Plant Science

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10.1111/mpp.12545

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Medina, C. A., Reyes, P. A., Trujillo, C. A., Gonzalez, J. L., Bejarano, D. A., Montenegro, N. A., Jacobs, J. M., Joe, A., Restrepo, S., Alfano, J. R., & Bernal, A. (2018). The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity. Molecular Plant Pathology, 19(3), 593-606. https://doi.org/10.1111/mpp.12545

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title = "The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity",

abstract = "Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) using heterologous systems. AvrBs2, XopR and XopAO1 are capable of suppressing PTI. ETI suppression activity was only detected for XopE4 and XopAO1. These results demonstrate the overall importance and diversity in functions of major virulence effectors AvrBs2 and XopAO1 in Xam during cassava infection.",

keywords = "ETI, PTI, type III effectors, virulence, Xanthomonas axonopodis pv. manihotis",

author = "Medina, {Cesar Augusto} and Reyes, {Paola Andrea} and Trujillo, {Cesar Augusto} and Gonzalez, {Juan Luis} and Bejarano, {David Alejandro} and Montenegro, {Nathaly Andrea} and Jacobs, {Jonathan M.} and Anna Joe and Silvia Restrepo and Alfano, {James R.} and Adriana Bernal",

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year = "2018",

month = mar,

doi = "10.1111/mpp.12545",

language = "English (US)",

volume = "19",

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T1 - The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity

AU - Medina, Cesar Augusto

AU - Reyes, Paola Andrea

AU - Trujillo, Cesar Augusto

AU - Gonzalez, Juan Luis

AU - Bejarano, David Alejandro

AU - Montenegro, Nathaly Andrea

AU - Jacobs, Jonathan M.

AU - Joe, Anna

AU - Restrepo, Silvia

AU - Alfano, James R.

AU - Bernal, Adriana

PY - 2018/3

Y1 - 2018/3

N2 - Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) using heterologous systems. AvrBs2, XopR and XopAO1 are capable of suppressing PTI. ETI suppression activity was only detected for XopE4 and XopAO1. These results demonstrate the overall importance and diversity in functions of major virulence effectors AvrBs2 and XopAO1 in Xam during cassava infection.

AB - Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) using heterologous systems. AvrBs2, XopR and XopAO1 are capable of suppressing PTI. ETI suppression activity was only detected for XopE4 and XopAO1. These results demonstrate the overall importance and diversity in functions of major virulence effectors AvrBs2 and XopAO1 in Xam during cassava infection.

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The role of type III effectors from Xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity

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