The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

Janice C. Pedersen; Donald L. Reynolds; A. Ali

doi:10.2307/1593111

The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

Janice C. Pedersen, Donald L. Reynolds, A. Ali

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 10^0.25 mean tissue culture infective dose (TCID₅₀) and 10^-0.44 TCID₅₀ of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.

Original language	English (US)
Pages (from-to)	681-685
Number of pages	5
Journal	Avian diseases
Volume	44
Issue number	3
DOIs	https://doi.org/10.2307/1593111
State	Published - 2000
Externally published	Yes

Keywords

Avian pneumovirus
Reverse transcription-polymerase chain reaction
Swollen head syndrome
Turkey rhinotracheitis

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
General Immunology and Microbiology

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10.2307/1593111

Cite this

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title = "The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)",

abstract = "A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 100.25 mean tissue culture infective dose (TCID50) and 10-0.44 TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.",

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author = "Pedersen, {Janice C.} and Reynolds, {Donald L.} and A. Ali",

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T1 - The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

AU - Pedersen, Janice C.

AU - Reynolds, Donald L.

AU - Ali, A.

PY - 2000

Y1 - 2000

N2 - A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 100.25 mean tissue culture infective dose (TCID50) and 10-0.44 TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.

AB - A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 100.25 mean tissue culture infective dose (TCID50) and 10-0.44 TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.

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KW - Reverse transcription-polymerase chain reaction

KW - Swollen head syndrome

KW - Turkey rhinotracheitis

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The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

Abstract

Keywords

ASJC Scopus subject areas

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