Abstract
A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 100.25 mean tissue culture infective dose (TCID50) and 10-0.44 TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.
Original language | English (US) |
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Pages (from-to) | 681-685 |
Number of pages | 5 |
Journal | Avian diseases |
Volume | 44 |
Issue number | 3 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Avian pneumovirus
- Reverse transcription-polymerase chain reaction
- Swollen head syndrome
- Turkey rhinotracheitis
ASJC Scopus subject areas
- Food Animals
- Animal Science and Zoology
- General Immunology and Microbiology