The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

Janice C. Pedersen, Donald L. Reynolds, A. Ali

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 100.25 mean tissue culture infective dose (TCID50) and 10-0.44 TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.

Original languageEnglish (US)
Pages (from-to)681-685
Number of pages5
JournalAvian diseases
Volume44
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Avian pneumovirus
  • Reverse transcription-polymerase chain reaction
  • Swollen head syndrome
  • Turkey rhinotracheitis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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