The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv

Anna Moroni, Carlo Viscomi, Vanessa Sangiorgio, Cinzia Pagliuca, Tobias Meckel, Ferenc Horvath, Sabrina Gazzarrini, Paola Valbuzzi, James L. Van Etten, Dario DiFrancesco, Gerhard Thiel

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalFEBS Letters
Volume530
Issue number1-3
DOIs
StatePublished - Oct 23 2002

Keywords

  • Chlorella virus PBCV-1
  • HEK 293 cell
  • K channel
  • Kcv

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'The short N-terminus is required for functional expression of the virus-encoded miniature K<sup>+</sup> channel Kcv'. Together they form a unique fingerprint.

  • Cite this

    Moroni, A., Viscomi, C., Sangiorgio, V., Pagliuca, C., Meckel, T., Horvath, F., Gazzarrini, S., Valbuzzi, P., Van Etten, J. L., DiFrancesco, D., & Thiel, G. (2002). The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. FEBS Letters, 530(1-3), 65-69. https://doi.org/10.1016/S0014-5793(02)03397-5