The structure and function of replication protein A in DNA replication

Aishwarya Prakash, Gloria E.O. Borgstahl

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.

Original languageEnglish (US)
Pages (from-to)171-196
Number of pages26
JournalSub-cellular biochemistry
StatePublished - 2012


  • DNA replication
  • G-quadruplex
  • OB-fold
  • Protein-protein interaction
  • Replication protein A
  • Single-stranded DNA binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology
  • Cancer Research


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