TY - JOUR
T1 - The structure and function of replication protein A in DNA replication
AU - Prakash, Aishwarya
AU - Borgstahl, Gloria E.O.
N1 - Publisher Copyright:
© Springer Science+Business Media Dordrecht 2012.
PY - 2012
Y1 - 2012
N2 - In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.
AB - In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.
KW - DNA replication
KW - G-quadruplex
KW - OB-fold
KW - Protein-protein interaction
KW - Replication protein A
KW - Single-stranded DNA binding protein
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U2 - 10.1007/978-94-007-4572-8_10
DO - 10.1007/978-94-007-4572-8_10
M3 - Article
C2 - 22918586
AN - SCOPUS:84894489800
SN - 0306-0225
VL - 62
SP - 171
EP - 196
JO - Sub-cellular biochemistry
JF - Sub-cellular biochemistry
ER -