TY - CHAP
T1 - The Study of Small RNA Sequencing from Biological Samples (miRNA-seq)
AU - Elbezanti, Weam Othman
AU - Challagundla, Kishore B.
AU - Pandey, Manoj K.
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - Small RNA sequencing (sRNA-seq) has greatly transformed the study of molecular biology by allowing thorough analysis of several types of short RNA molecules, such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs). This chapter presents a comprehensive methodology for conducting miRNA -seq on biological materials. The text introduces the principles and uses of miRNA-seq, emphasizing its importance in gene regulation, disease processes, and therapeutic interventions. The chapter subsequently explores the essential stages of miRNA-seq, encompassing sample acquisition, RNA isolation, library construction, sequencing, and data interpretation. Every part contains pragmatic suggestions, guidance for resolving issues, and optimal methods to guarantee excellent outcomes. Upon completion of this chapter, readers will possess a comprehensive comprehension of the miRNA-seq workflow and will be adequately prepared to apply this potent approach in their own research endeavors.
AB - Small RNA sequencing (sRNA-seq) has greatly transformed the study of molecular biology by allowing thorough analysis of several types of short RNA molecules, such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs). This chapter presents a comprehensive methodology for conducting miRNA -seq on biological materials. The text introduces the principles and uses of miRNA-seq, emphasizing its importance in gene regulation, disease processes, and therapeutic interventions. The chapter subsequently explores the essential stages of miRNA-seq, encompassing sample acquisition, RNA isolation, library construction, sequencing, and data interpretation. Every part contains pragmatic suggestions, guidance for resolving issues, and optimal methods to guarantee excellent outcomes. Upon completion of this chapter, readers will possess a comprehensive comprehension of the miRNA-seq workflow and will be adequately prepared to apply this potent approach in their own research endeavors.
KW - DNA polymerase
KW - RNA-induced silencing complex (RISC)
KW - SYBR stain
KW - Small RNA
KW - microRNA
UR - http://www.scopus.com/inward/record.url?scp=85209701710&partnerID=8YFLogxK
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U2 - 10.1007/978-1-0716-4192-7_10
DO - 10.1007/978-1-0716-4192-7_10
M3 - Chapter
C2 - 39546203
AN - SCOPUS:85209701710
T3 - Methods in Molecular Biology
SP - 175
EP - 188
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -