The transcriptome of human oocytes

Arif Murat Kocabas, Javier Crosby, Pablo J. Ross, Hasan H. Otu, Zeki Beyhan, Handan Can, Wai Leong Tam, Guilherme J.M. Rosa, Robert G. Halgren, Bing Lim, Emilio Fernandez, Jose Bernardo Cibelli

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

The identification of genes and deduced pathways from the mature human oocyte can help us better understand oogenesis, folliculogenesis, fertilization, and embryonic development. Human metaphase II oocytes were used within minutes after removal from the ovary, and its transcriptome was compared with a reference sample consisting of a mixture of total RNA from 10 different normal human tissues not including the ovary. RNA amplification was performed by using a unique protocol. Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays were used for hybridizations. Compared with reference samples, there were 5,331 transcripts significantly up-regulated and 7,074 transcripts significantly down-regulated in the oocyte. Of the oocyte up-regulated probe sets, 1,430 have unknown function. A core group of 66 transcripts was identified by intersecting significantly up-regulated genes of the human oocyte with those from the mouse oocyte and from human and mouse embryonic stem cells. GeneChip array results were validated using RT-PCR in a selected set of oocyte-specific genes. Within the up-regulated probe sets, the top overrepresented categories were related to RNA and protein metabolism, followed by DNA metabolism and chromatin modification. This report provides a comprehensive expression baseline of genes expressed in in vivo matured human oocytes. Further understanding of the biological role of these genes may expand our knowledge on meiotic cell cycle, fertilization, chromatin remodeling, lineage commitment, pluripotency, tissue regeneration, and morphogenesis.

Original languageEnglish (US)
Pages (from-to)14027-14032
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number38
DOIs
StatePublished - Sep 19 2006
Externally publishedYes

Keywords

  • Genechip
  • Microarray
  • Pluripotency
  • RNA amplification
  • RT-PCR

ASJC Scopus subject areas

  • General

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