The Use of Two-Dimensional Gas Chromatography–Time-of-Flight Mass Spectrometry (GC×GC–TOF-MS) for Metabolomic Analysis of Polar Metabolites

Kimberly Ralston-Hooper, Amber Jannasch, Jiri Adamec, Maria Sepúlveda

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Scopus citations

Abstract

Metabolites produced by an organism can be quite extensive, and one analytical technique alone is not capable of their comprehensive detection and identification. The majority of environmental metabolomic studies have implemented proton nuclear magnetic resonance (1H-NMR) spectroscopy with little attention given to mass spectrometry (MS) techniques. In this chapter, an analytical technique is outlined that incorporates two-dimensional gas chromatography–time-of-flight MS (GC×GC–TOF-MS) for the identification and quantification of polar metabolites.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-211
Number of pages7
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume708
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Two-dimensional gas chromatography
  • mass spectrometry
  • time of flight

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'The Use of Two-Dimensional Gas Chromatography–Time-of-Flight Mass Spectrometry (GC×GC–TOF-MS) for Metabolomic Analysis of Polar Metabolites'. Together they form a unique fingerprint.

Cite this