The viral genome in experimental murine coxsackievirus B3 myocarditis: A Northern blotting anaylsis

Ikutaro Okada, Akira Matsumori, Chuichi Kawai, Junji Yodoi, Steven Tracy

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The presence of viral genomes in the murine heart in experimental coxsackievirus B3 myocarditis was investigated by Northern blotting analysis. Four-week-old C3H He mice were inoculated with coxsackievirus B3. After sacrifice, the hearts were divided into 3 parts. Total RNA was extracted from one part of the heart. The other two parts were used for investigation of histopathology and of virus titer by plaque assay. The 32P-labeled cDNA probe was derived from the 5′ end sequence of the coxsackievirus B3 genome. Northern blot autoradiograms of heart RNA were positive for viral RNA until day 7 and negative after day 10 and in control (uninfected) hearts. Positive autoradiograms always had the strongest signal at about 7.4 kilobase, corresponding to the size of the complete genome of the virus. The viral genomes were detected earlier than the appearance of the histopathologic changes in the murine heart. This type analysis of the viral genome in the murine myocardium may be useful in evaluating the effects of specific drugs on experimental viral myocarditis at the level of the viral genome.

Original languageEnglish (US)
Pages (from-to)999-1008
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume22
Issue number9
DOIs
StatePublished - Sep 1990

Keywords

  • Coxsackievirus B3
  • Murine myocarditis
  • Northern blotting
  • Viral genome
  • cDNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'The viral genome in experimental murine coxsackievirus B3 myocarditis: A Northern blotting anaylsis'. Together they form a unique fingerprint.

  • Cite this