Thermal processing of peanut impacts detection by current analytical techniques

Justin T. Marsh, Shyamali Jayasena, Ferdelie Gaskin, Joseph L. Baumert, Philip Johnson

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Recalls of spice containing products due to undeclared peanut have highlighted the importance of analytical methods in these foods. We examined the performance of peanut detection methods in cumin and garlic, focusing on quantitative ELISA. Although suitable for qualitative detection, accurate quantitation proved difficult. Roasting of peanut contaminants influenced ELISA results, with raw peanut over-detected (3.9-fold) and roasted peanut under-detected (3.5-fold). Further investigation demonstrated the importance of protein targets for ELISA. The kit which gave the least variable results was based on detection of 2S albumin proteins. Additionally, we show that these proteins are more efficiently extracted from roasted peanut. We conclude that current methods are largely suitable for the qualitative detection of peanut in cumin and garlic. Quantitation relies on assumptions as to the state of thermal processing of peanut. We suggest that analytical method providers address robust detection by target selection, including identifying targets by MS.

Original languageEnglish (US)
Article number126019
JournalFood Chemistry
StatePublished - May 30 2020


  • Allergen
  • Arachis hypogaea
  • Food allergy
  • Mass spectrometry
  • Peanut
  • Spice

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science


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