Three-dimensional tissues using Human pluripotent stem cell spheroids as biofabrication building blocks

Haishuang Lin, Qiang Li, Yuguo Lei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

A recently emerged approach for tissue engineering is tobiofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can becultured togenerate large numbers ofcells and can presumably be differentiated into all the cell types of the human body in vitro, thus are an ideal cell source for biofabrication. Wepreviously developed ahydrogel-based cell culture system that can economically produce large numbers of hPSC spheroids. With hPSCs and this culture system, there are two potential methods tobiofabricate adesired tissue. In Method 1, hPSC spheroids arefirst utilized tobiofabricate an hPSC tissue thatis subsequently differentiated into the desired tissue. In Method 2, hPSC spheroids are first converted into tissue spheroids in the hydrogel-based culture system and the tissue spheroids are then utilized tobiofabricate the desired tissue.Inthis paper, we systematically measured the fusion rates ofhPSC spheroids without and with differentiation toward cortical and midbrain dopaminergic neurons and found spheroids'fusion rates dropped sharply as differentiation progressed. We found Method 1 was appropriate for biofabricating neural tissues.

Original languageEnglish (US)
Title of host publicationMaterials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting
PublisherAIChE
Pages863-879
Number of pages17
ISBN (Electronic)9781510857971
StatePublished - 2017
EventMaterials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting - Minneapolis, United States
Duration: Oct 29 2017Nov 3 2017

Publication series

NameMaterials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting
Volume2

Other

OtherMaterials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting
CountryUnited States
CityMinneapolis
Period10/29/1711/3/17

Keywords

  • Human pluripotent stem cells
  • Organ bioprinting
  • Spheroids
  • Tissue biofabrication

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)
  • Materials Science(all)

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  • Cite this

    Lin, H., Li, Q., & Lei, Y. (2017). Three-dimensional tissues using Human pluripotent stem cell spheroids as biofabrication building blocks. In Materials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting (pp. 863-879). (Materials Engineering and Sciences Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting; Vol. 2). AIChE.