Thyroid hormone receptors TRα1 and TRβ differentially regulate gene expression of Kcnq4 and prestin during final differentiation of outer hair cells

Herald Winter, Claudia Braig, Ulrike Zimmermann, Hyun Soon Geisler, Jürgen Theodor Fränzer, Thomas Weber, Matthias Ley, Jutta Engel, Martina Knirsch, Karl Bauer, Stephanie Christ, Edward J. Walsh, JoAnn McGee, Iris Köpschall, Karin Rohbock, Marlies Knipper

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Thyroid hormone (TH or T3) and TH-receptor β (TRβ) have been reported to be relevant for cochlear development and hearing function. Mutations in the TRβ gene result in deafness associated with resistance to TH syndrome. The effect of TRα1 on neither hearing function nor cochlear T3 target genes has been described to date. It is also uncertain whether TRα1 and TRβ can act simultaneously on different target genes within a single cell. We focused on two concomitantly expressed outer hair cell genes, the potassium channel Kcnq4 and the motor protein prestin Slc26a5. In outer hair cells, TH enhanced the expression of the prestin gene through TRβ. Simultaneously Kcnq4 expression was activated in the same cells by derepression of TRα1 aporeceptors mediated by an identified TH-response element, which modulates KCNQ4 promoter activity. We show that T3 target genes can differ in their sensitivity to TH receptors having the ligand either bound (holoreceptors) or not bound (aporeceptors) within single cells, and suggest a role for TRα1 in final cell differentiation.

Original languageEnglish (US)
Pages (from-to)2975-2984
Number of pages10
JournalJournal of cell science
Volume119
Issue number14
DOIs
StatePublished - Jul 15 2006

Keywords

  • Final differentiation
  • KCNQ4
  • Prestin
  • TR aporeceptors

ASJC Scopus subject areas

  • Cell Biology

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    Winter, H., Braig, C., Zimmermann, U., Geisler, H. S., Fränzer, J. T., Weber, T., Ley, M., Engel, J., Knirsch, M., Bauer, K., Christ, S., Walsh, E. J., McGee, J., Köpschall, I., Rohbock, K., & Knipper, M. (2006). Thyroid hormone receptors TRα1 and TRβ differentially regulate gene expression of Kcnq4 and prestin during final differentiation of outer hair cells. Journal of cell science, 119(14), 2975-2984. https://doi.org/10.1242/jcs.03013