Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness

Zahra Goli-Malekabadi, Mohammad Tafazzoli-Shadpour, Ali Tamayol, Ehsan Seyedjafari

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness. Methods: We monitored cell spreading, internal structure, 3D topography, and the viability of ECs over 24 hours of culture on polydimethylsiloxane (PDMS) substrates with two different degrees of elastic modulus. Results: Despite significant differences in cell spreading after cell seeding, cells showed a similar shape and internal structure after 24 hours of culture on both soft and stiff substrates. However, 3D topographical images confirmed existence of rich lamellipodia and filopodia around the cells cultured on stiffer PDMS substrates. Conclusion: It was concluded that the response of ECs to the substrate stiffness was time dependent with initial enhanced cellular spreading and viability on stiffer substrates. Results can provide a better comprehension of cell mechanotransduction for tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalBioImpacts
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2017

Keywords

  • 3D topography
  • HUVECs
  • Morphology
  • Substrate stiffness
  • Time dependency

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmaceutical Science

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