Tissue-engineered small intestine improves recovery after massive small bowel resection

Tracy C. Grikscheit, Aleem Siddique, Erin R. Ochoa, Ashok Srinivasan, Eben Alsberg, Richard A. Hodin, Joseph P. Vacanti

Research output: Contribution to journalArticlepeer-review

194 Scopus citations


Objective: Rescue with tissue-engineered small intestine (TESI) after massive small bowel resection (MSBR). Summary Background Data: Short bowel syndrome is a morbid product of massive small bowel resection. We report the first replacement of a vital organ by tissue engineering with TESI after MSBR. Methods: Ten male Lewis rats underwent TESI implantation with green fluorescent protein (GFP)-marked cells (TESI+, n = 5) or sham laparotomy (TESI-, n = 5) followed by MSBR. Side-to-side anastomosis of TESI to proximal small intestine was performed or omitted. TESIØ animals underwent implantation of engineered intestine with no further surgery. Weights were measured QOD until day 40. Transit times were measured. DNA assay was performed with computer morphometry. Northern blots of RNA were probed for intestinal alkaline phosphatase (IAP) and villin. Hematoxylin and eosin, S100, and smooth muscle actin immunohistochemistry were performed. Blood was collected at sacrifice. Results: All 10 rats initially lost then regained weight. The initial rate of weight loss was higher in TESI+ versus TESI-, but the nadir was reached a week earlier with more rapid weight gain subsequently to 98% preoperative weight on day 40 in animals with engineered intestine versus 76% (P < 0.03). Serum B12 was higher at 439 pg/mL versus 195.4 pg/mL. IAP mRNA appeared greater in TESI+ than TESIØ, with constant villin levels. Histology revealed appropriate architecture including nerve. GFP labeling persisted. Conclusions: Anastomosis of TESI significantly improved postoperative weight and B12 absorption after MSBR. IAP, a marker of differentiation in intestinal epithelium, is present in TESI, and GFP labeling was accomplished.

Original languageEnglish (US)
Pages (from-to)748-754
Number of pages7
JournalAnnals of surgery
Issue number5
StatePublished - Nov 2004
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


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