Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

Steven D. Carson; Philip G. Archer

doi:10.1016/0049-3848(86)90228-8

Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

Steven D. Carson, Philip G. Archer

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.

Original language	English (US)
Pages (from-to)	185-195
Number of pages	11
Journal	Thrombosis Research
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/0049-3848(86)90228-8
State	Published - Jan 15 1986
Externally published	Yes

Keywords

cell culture
cell density
chromogenic assay
tissue factor

ASJC Scopus subject areas

Hematology

Access to Document

10.1016/0049-3848(86)90228-8

Cite this

@article{29281bed048041ca9854538d4aa68760,

title = "Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader",

abstract = "Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.",

keywords = "cell culture, cell density, chromogenic assay, tissue factor",

author = "Carson, {Steven D.} and Archer, {Philip G.}",

note = "Funding Information: This work was supported by grants HL-31408 and BRSG-05357 from NIH and an award from the Colorado Heart Association; departmental facilities were supported by a gift from R.J. Reynolds Industries, Inc., and by grant CA-15823 from NIH.",

year = "1986",

month = jan,

day = "15",

doi = "10.1016/0049-3848(86)90228-8",

language = "English (US)",

volume = "41",

pages = "185--195",

journal = "Thrombosis Research",

issn = "0049-3848",

publisher = "Elsevier Ltd",

number = "2",

}

TY - JOUR

T1 - Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

AU - Carson, Steven D.

AU - Archer, Philip G.

N1 - Funding Information: This work was supported by grants HL-31408 and BRSG-05357 from NIH and an award from the Colorado Heart Association; departmental facilities were supported by a gift from R.J. Reynolds Industries, Inc., and by grant CA-15823 from NIH.

PY - 1986/1/15

Y1 - 1986/1/15

N2 - Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.

AB - Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.

KW - cell culture

KW - cell density

KW - chromogenic assay

KW - tissue factor

UR - http://www.scopus.com/inward/record.url?scp=0022618159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022618159&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(86)90228-8

DO - 10.1016/0049-3848(86)90228-8

M3 - Article

C2 - 3515617

AN - SCOPUS:0022618159

SN - 0049-3848

VL - 41

SP - 185

EP - 195

JO - Thrombosis Research

JF - Thrombosis Research

IS - 2

ER -

Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this