Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain

S. D. Carson, M. E. Bromberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.

Original languageEnglish (US)
Pages (from-to)657-663
Number of pages7
JournalThrombosis and Haemostasis
Volume84
Issue number4
StatePublished - Jan 1 2000

Keywords

  • Cytoplasmic domain
  • Encryption
  • Tissue factor

ASJC Scopus subject areas

  • Hematology

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