TNF-α and -β gene polymorphisms in multiple sclerosis: A highly significant role for determinants in the first intron of the TNF-β gene

Jose Americo M.Fernandes Filho, Christian A. Vedeler, Kjell Morten Myhr, Harald Nyland, Janardan P. Pandey

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Tumor necrosis factor (TNF)-α and TNF-β are proinflammatory cytokines that have been implicated in the pathogenesis of several autoimmune diseases. The aim of this investigation was to determine whether a determinant in the first intron of the TNF-β gene (TNF-β+252) and two promoter-region polymorphisms of the TNF-α gene (TNF-α-308 and TNF-α-238) affect susceptibility to multiple sclerosis (MS). DNA samples from 133 Caucasian MS patients and 148 healthy controls from Norway were genotyped for several polymorphic determinants, using polymerase chain reaction-based restriction fragment length polymorphisms (PCR-RFLP) methods. TNF-β+252 genotypes were significantly associated with MS: The frequency of TNF-β 2,2 was increased (p = 0.00009) while the frequency of TNF-β 1,2 was decreased (p = 0.0012) in MS patients as compared to controls. TNF-α genotypes were not associated with MS. These results suggest that the TNF-β gene plays a significant role in the etiopathogenesis of MS.

Original languageEnglish (US)
Pages (from-to)377-380
Number of pages4
JournalAutoimmunity
Volume35
Issue number6
DOIs
StatePublished - Sep 2002
Externally publishedYes

Keywords

  • Intron
  • Multiple sclerosis
  • Polymorphism
  • Proinflammatory cytokines
  • TNF-α
  • TNF-β

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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