TNT nitroreductase from a Pseudomonas aeruginosa strain isolated from TNT-contaminated soil

B. T. Oh, G. Sarath, P. J. Shea

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Microbial degradation and detoxification of TNT (2,4,6-trinitrotoluene) in soils ultimately depends on the presence of effective intracellular or extracellular enzymes. TNT was rapidly degraded by an enzyme obtained from a Pseudomonas aeruginosa strain isolated from TNT-contaminated soil. The nitroreductase enzyme was purified to apparent electrophoretic homogeneity by sequential chromatography on phenyl-sepharose, hydroxyapatite, and ion exchange resins. The TNT-nitroreductase catalyzed reduction of TNT to 4-hydroxylamino-4,6-dinitrotoluene (4HADNT) and 4-amino-2,6-dinitrotoluene (4ADNT). TNT reduction to 4ADNT in cell-free enzyme preparations corresponded to 2ADNT production by intact cells. The enzyme required NADH as a cosubstrate and the optimal pH for the reaction was approximately 6.5. The purified enzyme also exhibited NADH diaphorase activity and its denatured molecular weight was approximately 54 kDa.

Original languageEnglish (US)
Pages (from-to)875-881
Number of pages7
JournalSoil Biology and Biochemistry
Volume33
Issue number7-8
DOIs
StatePublished - 2001

Keywords

  • Bacteria
  • Enzyme
  • Explosive
  • Reduction

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

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