Top-Down Proteomics

C. Boone, J. Adamec

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Top-Down approach refers to the ionization of intact proteins and MS or MS/MS analysis of intact species or fragment ions generated in mass spectrometer by induced dissociation. This tehnique can eliminate most of the problems associated with the Bottom-Up approach and allow for high specificity protein identification and characterization of different proteoforms: information that would otherwise be lost with protein digestion and unattainable with other large scale, whole proteome approaches. Major obstacle problem in Top-Down proteomics represent sample complexity - biological samples are inherently complex with a multitude of different proteins and their proteoforms; thus, effective implementation of the Top-Down approach requires extensive separation and/or enrichment step preceding mass analysis. In this chapter, we will first describe methods of used for fractionation of intact proteins. Following that, we will discuss ionization techniques and instrumentation used in Top-Down proteomics.

Original languageEnglish (US)
Title of host publicationProteomic Profiling and Analytical Chemistry
Subtitle of host publicationThe Crossroads: Second Edition
PublisherElsevier Inc.
Pages175-191
Number of pages17
ISBN (Print)9780444636881
DOIs
StatePublished - Mar 22 2016

Keywords

  • Biological sample
  • Data analysis
  • Ionization techniques
  • Mass spectrometry
  • Protein separation
  • Proteoforms
  • Sample complexity
  • Top-down proteomics

ASJC Scopus subject areas

  • Chemistry(all)

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  • Cite this

    Boone, C., & Adamec, J. (2016). Top-Down Proteomics. In Proteomic Profiling and Analytical Chemistry: The Crossroads: Second Edition (pp. 175-191). Elsevier Inc.. https://doi.org/10.1016/B978-0-444-63688-1.00010-0