Towards normalization of soybean somatic embryo maturation

M. A. Schmidt, D. M. Tucker, E. B. Cahoon, W. A. Parrott

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal salt formulations were tested in an effort to modify existing soybean embryogenesis histodifferentiation/maturation media to further normalize the development of soybean somatic embryos. The resultant liquid medium, referred to as soybean histodifferentiation and maturation medium (SHaM), consists of FNL basal salts, 3% sucrose, 3% sorbitol, filter-sterilized 30 mM glutamine and 1 mM methionine. SHaM-derived somatic embryos are more similar to seed in terms of protein and fatty acid/lipid composition, and conversion ability, than somatic embryos obtained from traditional soybean histodifferentiation and maturation media.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalPlant Cell Reports
Volume24
Issue number7
DOIs
StatePublished - Sep 2005

Keywords

  • Glutamine
  • Glycine max
  • Methionine
  • Oil
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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