Toxicity and composition of protease-inhibited Bacillus thuringiensis var. israelensis crystals

Mary Ann Pfannenstiel; Elise J. Ross; Vance C. Kramer; Kenneth W. Nickerson

doi:10.1111/j.1574-6968.1984.tb00182.x

Toxicity and composition of protease-inhibited Bacillus thuringiensis var. israelensis crystals

Mary Ann Pfannenstiel, Elise J. Ross, Vance C. Kramer, Kenneth W. Nickerson

Biological Sciences

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

A procedure is described in which the protein crystals produced by Bacillus thuringiensis var. israelensis were solubilized in 50 mM NaOH with 10 mM EDTA at pH 11.7. This solubilization procedure gave protein gel profiles identical with those for intact crystals while maintaining full biological activity in the form of erythrocyte lysis capability. Crystals with and without protease activity were equally toxic to Aedes aegypti larvae.

Original language	English (US)
Pages (from-to)	39-42
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	21
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1984.tb00182.x
State	Published - Jan 1984

Keywords

Aedes aegypti larvae
Bacillus thuringiensis var. israelensis
crystal solubilization
hemolysis
metalloprotease
mosquito toxin

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1984.tb00182.x

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abstract = "A procedure is described in which the protein crystals produced by Bacillus thuringiensis var. israelensis were solubilized in 50 mM NaOH with 10 mM EDTA at pH 11.7. This solubilization procedure gave protein gel profiles identical with those for intact crystals while maintaining full biological activity in the form of erythrocyte lysis capability. Crystals with and without protease activity were equally toxic to Aedes aegypti larvae.",

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AU - Pfannenstiel, Mary Ann

AU - Ross, Elise J.

AU - Kramer, Vance C.

AU - Nickerson, Kenneth W.

PY - 1984/1

Y1 - 1984/1

N2 - A procedure is described in which the protein crystals produced by Bacillus thuringiensis var. israelensis were solubilized in 50 mM NaOH with 10 mM EDTA at pH 11.7. This solubilization procedure gave protein gel profiles identical with those for intact crystals while maintaining full biological activity in the form of erythrocyte lysis capability. Crystals with and without protease activity were equally toxic to Aedes aegypti larvae.

AB - A procedure is described in which the protein crystals produced by Bacillus thuringiensis var. israelensis were solubilized in 50 mM NaOH with 10 mM EDTA at pH 11.7. This solubilization procedure gave protein gel profiles identical with those for intact crystals while maintaining full biological activity in the form of erythrocyte lysis capability. Crystals with and without protease activity were equally toxic to Aedes aegypti larvae.

KW - Aedes aegypti larvae

KW - Bacillus thuringiensis var. israelensis

KW - crystal solubilization

KW - hemolysis

KW - metalloprotease

KW - mosquito toxin

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Toxicity and composition of protease-inhibited Bacillus thuringiensis var. israelensis crystals

Abstract

Keywords

ASJC Scopus subject areas

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