Transcriptional Profiling of Resistant and Susceptible Buffalograsses in Response to Blissus occiduus (Hemiptera: Blissidae) Feeding

Crystal Ramm, Michael Wachholtz, Keenan Amundsen, Teresa Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Fred Baxendale

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with susceptible control plants. Defense-related transcripts, including two peroxidase genes, two catalase genes, several cytochrome P450 transcripts, a glutathione s-transferase, and a WRKY gene were upregulated within the Prestige transcriptome in response to chinch bug feeding. The majority of observed transcripts with oxidoreductase activity, including nine peroxidase genes and a catalase gene, were downregulated in 378 in response to initial chinch bug feeding. The observed difference in transcript expression between these two buffalograss genotypes provides insight into the mechanism(s) of resistance, specifically buffalograss tolerance to chinch bug feeding.

Original languageEnglish (US)
Pages (from-to)1354-1362
Number of pages9
JournalJournal of economic entomology
Volume108
Issue number3
DOIs
StatePublished - Jun 1 2015

Keywords

  • buffalograss
  • catalase
  • chinch bug
  • peroxidase
  • transcriptome

ASJC Scopus subject areas

  • Ecology
  • Insect Science

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    Ramm, C., Wachholtz, M., Amundsen, K., Donze, T., Heng-Moss, T., Twigg, P., Palmer, N. A., Sarath, G., & Baxendale, F. (2015). Transcriptional Profiling of Resistant and Susceptible Buffalograsses in Response to Blissus occiduus (Hemiptera: Blissidae) Feeding. Journal of economic entomology, 108(3), 1354-1362. https://doi.org/10.1093/jee/tov067