Transcriptome analysis of the zebrafish mind bomb mutant

Junmo Hwang, Hyung Soo Kim, Ji Woong Seok, Jun Dae Kim, Soonil Koun, So Young Park, Jinseok Lee, Hyang Sook Kim, Hyung Seok Kim, Kil Soo Kim, Kyu Tae Chang, Zae Young Ryoo, San Ming Wang, Tae Lin Huh, Sanggyu Lee

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mibta52b mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mibta52b mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mibta52b mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mibta52b mutant as compared with the wild-type. Interestingly, it was suggested that the mibta52b mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalMolecular Genetics and Genomics
Volume281
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Genome-wide analysis
  • Mind bomb
  • SAGE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Hwang, J., Kim, H. S., Seok, J. W., Kim, J. D., Koun, S., Park, S. Y., Lee, J., Kim, H. S., Kim, H. S., Kim, K. S., Chang, K. T., Ryoo, Z. Y., Wang, S. M., Huh, T. L., & Lee, S. (2009). Transcriptome analysis of the zebrafish mind bomb mutant. Molecular Genetics and Genomics, 281(1), 77-85. https://doi.org/10.1007/s00438-008-0395-5