Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin

Paul H. Davis; Jochen Schulze; Samuel L. Stanley

doi:10.1016/j.molbiopara.2006.10.014

Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin

Paul H. Davis, Jochen Schulze, Samuel L. Stanley

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

The availability of Rahman, and the virulent HM-1:IMSS strain of E. histolytica, provides a powerful tool for identifying virulence factors of E. histolytica. Here we report an attempt to identify potential virulence factors of E. histolytica by comparing the transcriptome of E. histolytica HM-1:IMSS and E. histolytica Rahman. With phenotypically defined strains, we compared the transcriptome of Rahman and HM-1:IMSS using a custom 70mer oligonucleotide based microarray that has essentially full representation of the E. histolytica HM-1:IMSS genome. We find extensive differences between the two strains, including distinct patterns of gene expression of cysteine proteinases, AIG family members, and lectin light chains.

Original language	English (US)
Pages (from-to)	118-128
Number of pages	11
Journal	Molecular and Biochemical Parasitology
Volume	151
Issue number	1
DOIs	https://doi.org/10.1016/j.molbiopara.2006.10.014
State	Published - Jan 2007
Externally published	Yes

Keywords

E. histolytica
HM-1:IMSS
Proteomics
Rahman
Strain comparison
microarray

ASJC Scopus subject areas

Parasitology
Molecular Biology

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Davis, P. H., Schulze, J., & Stanley, S. L. (2007). Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. Molecular and Biochemical Parasitology, 151(1), 118-128. https://doi.org/10.1016/j.molbiopara.2006.10.014

Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. / Davis, Paul H.; Schulze, Jochen; Stanley, Samuel L.
In: Molecular and Biochemical Parasitology, Vol. 151, No. 1, 01.2007, p. 118-128.

Research output: Contribution to journal › Article › peer-review

Davis, PH, Schulze, J & Stanley, SL 2007, 'Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin', Molecular and Biochemical Parasitology, vol. 151, no. 1, pp. 118-128. https://doi.org/10.1016/j.molbiopara.2006.10.014

Davis PH, Schulze J, Stanley SL. Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. Molecular and Biochemical Parasitology. 2007 Jan;151(1):118-128. doi: 10.1016/j.molbiopara.2006.10.014

Davis, Paul H. ; Schulze, Jochen ; Stanley, Samuel L. / Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. In: Molecular and Biochemical Parasitology. 2007 ; Vol. 151, No. 1. pp. 118-128.

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abstract = "The availability of Rahman, and the virulent HM-1:IMSS strain of E. histolytica, provides a powerful tool for identifying virulence factors of E. histolytica. Here we report an attempt to identify potential virulence factors of E. histolytica by comparing the transcriptome of E. histolytica HM-1:IMSS and E. histolytica Rahman. With phenotypically defined strains, we compared the transcriptome of Rahman and HM-1:IMSS using a custom 70mer oligonucleotide based microarray that has essentially full representation of the E. histolytica HM-1:IMSS genome. We find extensive differences between the two strains, including distinct patterns of gene expression of cysteine proteinases, AIG family members, and lectin light chains.",

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Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin

Abstract

Keywords

ASJC Scopus subject areas

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