TY - JOUR
T1 - Transcriptomic variation of hepatopancreas reveals the energy metabolism and biological processes associated with molting in Chinese mitten crab, Eriocheir sinensis
AU - Huang, Shu
AU - Wang, Jun
AU - Yue, Wucheng
AU - Chen, Jiao
AU - Gaughan, Sarah
AU - Lu, Weiqun
AU - Lu, Guoqing
AU - Wang, Chenghui
N1 - Funding Information:
We would like to thank Yuanji Ci and Lian Wu for their assistance in RNA isolation and Professor Sheng Li for proof-reading the manuscript. We particularly thank the Holland Computing Center (HCC) at the University of Nebraska for computational support and technical assistance. This work was funded by Shanghai Technology System for Chinese Mitten Crab Industry (No. 2014-4), Shanghai Science and Technology Committee Programs (No. 13391912102; No. 03DZ2251800) and Innovation project of Shanghai Ocean University Graduate Education.
PY - 2015/9/15
Y1 - 2015/9/15
N2 - Molting is a critical developmental process for crustaceans, yet the underlying molecular mechanism is unknown. In this study, we used RNA-Seq to investigate transcriptomic profiles of the hepatopancreas and identified differentially expressed genes at four molting stages of Chinese mitten crab (Eriocheir sinensis). A total of 97,398 transcripts were assembled, with 31,900 transcripts annotated. Transcriptomic comparison revealed 1,189 genes differentially expressed amongst different molting stages. We observed a pattern associated with energy metabolism and physiological responses during a molting cycle. In specific, differentially expressed genes enriched in postmolt were linked to energy consumption whereas genes enriched in intermolt were related to carbohydrates, lipids metabolic and biosynthetic processes. In premolt, a preparation stage for upcoming molting and energy consumption, highly expressed genes were enriched in response to steroid hormone stimulus and immune system development. The expression profiles of twelve functional genes detected via RNA-Seq were corroborated through real-time RT-PCR assay. Together, our results, including assembled transcriptomes, annotated functional elements and enriched differentially expressed genes amongst different molting stages, provide novel insights into the functions of the hepatopancreas in energy metabolism and biological processes pertaining to molting in crustaceans.
AB - Molting is a critical developmental process for crustaceans, yet the underlying molecular mechanism is unknown. In this study, we used RNA-Seq to investigate transcriptomic profiles of the hepatopancreas and identified differentially expressed genes at four molting stages of Chinese mitten crab (Eriocheir sinensis). A total of 97,398 transcripts were assembled, with 31,900 transcripts annotated. Transcriptomic comparison revealed 1,189 genes differentially expressed amongst different molting stages. We observed a pattern associated with energy metabolism and physiological responses during a molting cycle. In specific, differentially expressed genes enriched in postmolt were linked to energy consumption whereas genes enriched in intermolt were related to carbohydrates, lipids metabolic and biosynthetic processes. In premolt, a preparation stage for upcoming molting and energy consumption, highly expressed genes were enriched in response to steroid hormone stimulus and immune system development. The expression profiles of twelve functional genes detected via RNA-Seq were corroborated through real-time RT-PCR assay. Together, our results, including assembled transcriptomes, annotated functional elements and enriched differentially expressed genes amongst different molting stages, provide novel insights into the functions of the hepatopancreas in energy metabolism and biological processes pertaining to molting in crustaceans.
UR - http://www.scopus.com/inward/record.url?scp=84941670537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941670537&partnerID=8YFLogxK
U2 - 10.1038/srep14015
DO - 10.1038/srep14015
M3 - Article
C2 - 26369734
AN - SCOPUS:84941670537
VL - 5
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 14015
ER -