Transduction of cellular sequence by a human immunodeficiency virus type 1-derived vector

G. Sun, P. K. O'Neil, H. Yu, Y. Ron, B. D. Preston, J. P. Dougherty

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

During studies examining the rate of human immunodeficiency virus type 1 (HIV-1) mutation in a single cycle of replication, the 5′ long terminal repeat of one progeny provirus was found to contain an insertion of 147 bp including an entire tRNA3Lys sequence as well as an additional 66 bp insertion of nonviral origin. Database searches revealed that 65 of 66 bp aligned with the human CpG island sequence found on chromosomes 6, 14, and 17. Therefore it seems probable that it is of human cellular sequence origin and was transduced by HIV-1. This is the first demonstration that HIV-1 can capture a cellular sequence. The site of integration of the parental provirus was mapped to chromosome 1p32.1. Sequence with homology to the transduced CpG island was not found on chromosome 1, suggesting that the transduced cellular sequence was not linked to the site of viral integration.

Original languageEnglish (US)
Pages (from-to)11902-11906
Number of pages5
JournalJournal of virology
Volume75
Issue number23
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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