Transduction of hematopoietic stem cells in humans and in nonhuman primates

Cynthia E. Dunbar, John Tisdale, Jian Mei Yu, Toshihiro Soma, Joanne Zujewski, David Bodine, Stephanie Sellers, Ken Cowan, Robert Donahue, Robert Emmons

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Primitive hematopoietic progenitor and stem cells have been pursued as highly desirable targets for genetic therapy. Retroviral vectors have been used for the majority of preclinical and clinical studies directed at these cells; however, both preclinical and early clinical studies indicate that the gene transfer efficiency of the current generation of vectors using known transduction conditions into primate and human repopulating stem cells is too low to be of clinical utility in most situations. In this presentation I will summarize the status of our completed and ongoing clinical genetic marking trials, and describe our efforts in the laboratory and use of primate transplantation models to improve on these results.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalSTEM CELLS
Volume15
Issue numberSUPPL. 1
DOIs
StatePublished - 1997

Keywords

  • Autologous transplantation
  • Gene therapy
  • Gene transfer
  • Hematopoiesis
  • Retrovirus
  • Stem cell
  • Stem cell factor
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Dunbar, C. E., Tisdale, J., Yu, J. M., Soma, T., Zujewski, J., Bodine, D., Sellers, S., Cowan, K., Donahue, R., & Emmons, R. (1997). Transduction of hematopoietic stem cells in humans and in nonhuman primates. STEM CELLS, 15(SUPPL. 1), 135-140. https://doi.org/10.1002/stem.5530150817