Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu; Elisabeth J. Chapman; Zhiyong Yang; James C. Carrington; Xuemei Chen

doi:10.1016/j.febslet.2006.04.063

Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu, Elisabeth J. Chapman, Zhiyong Yang, James C. Carrington, Xuemei Chen

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA^* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA^* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA^* duplexes in vivo. These findings suggest miRNA/miRNA^* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

Original language	English (US)
Pages (from-to)	3117-3120
Number of pages	4
Journal	FEBS Letters
Volume	580
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.063
State	Published - May 29 2006
Externally published	Yes

Keywords

HEN1
Methylation
Viral RNA silencing suppressor
microRNA/microRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis",

abstract = "HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.",

keywords = "HEN1, Methylation, Viral RNA silencing suppressor, microRNA/microRNA",

author = "Bin Yu and Chapman, {Elisabeth J.} and Zhiyong Yang and Carrington, {James C.} and Xuemei Chen",

note = "Funding Information: This work was supported by NSF (MCB-0612958 to X.C. and MCB-0209836 to J.C.). ",

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T1 - Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

AU - Yu, Bin

AU - Chapman, Elisabeth J.

AU - Yang, Zhiyong

AU - Carrington, James C.

AU - Chen, Xuemei

N1 - Funding Information: This work was supported by NSF (MCB-0612958 to X.C. and MCB-0209836 to J.C.).

PY - 2006/5/29

Y1 - 2006/5/29

N2 - HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

AB - HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

KW - HEN1

KW - Methylation

KW - Viral RNA silencing suppressor

KW - microRNA/microRNA

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Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Abstract

Keywords

ASJC Scopus subject areas

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