Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu; Elisabeth J. Chapman; Zhiyong Yang; James C. Carrington; Xuemei Chen

doi:10.1016/j.febslet.2006.04.063

Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu, Elisabeth J. Chapman, Zhiyong Yang, James C. Carrington, Xuemei Chen

Biological Sciences

Research output: Contribution to journal › Article

84 Scopus citations

Abstract

HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA^* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA^* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA^* duplexes in vivo. These findings suggest miRNA/miRNA^* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

Original language	English (US)
Pages (from-to)	3117-3120
Number of pages	4
Journal	FEBS Letters
Volume	580
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.063
State	Published - May 29 2006

Keywords

HEN1
Methylation
Viral RNA silencing suppressor
microRNA/microRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Yu, B., Chapman, E. J., Yang, Z., Carrington, J. C., & Chen, X. (2006). Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis. FEBS Letters, 580(13), 3117-3120. https://doi.org/10.1016/j.febslet.2006.04.063

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