Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

D. D. Songstad, F. G. Halaka, D. L. DeBoer, C. L. Armstrong, M. A.W. Hinchee, C. G. Ford-Santino, S. M. Brown, M. E. Fromm, R. B. Horsch

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume33
Issue number2
DOIs
StatePublished - May 1993
Externally publishedYes

Keywords

  • DNA delivery
  • Zea mays
  • electroporation electrolyte
  • heat shock

ASJC Scopus subject areas

  • Horticulture

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    Songstad, D. D., Halaka, F. G., DeBoer, D. L., Armstrong, C. L., Hinchee, M. A. W., Ford-Santino, C. G., Brown, S. M., Fromm, M. E., & Horsch, R. B. (1993). Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. Plant Cell, Tissue and Organ Culture, 33(2), 195-201. https://doi.org/10.1007/BF01983234