Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

D. D. Songstad; F. G. Halaka; D. L. DeBoer; C. L. Armstrong; M. A.W. Hinchee; C. G. Ford-Santino; S. M. Brown; M. E. Fromm; R. B. Horsch

doi:10.1007/BF01983234

Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

D. D. Songstad, F. G. Halaka, D. L. DeBoer, C. L. Armstrong, M. A.W. Hinchee, C. G. Ford-Santino, S. M. Brown, M. E. Fromm, R. B. Horsch

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.

Original language	English (US)
Pages (from-to)	195-201
Number of pages	7
Journal	Plant Cell, Tissue and Organ Culture
Volume	33
Issue number	2
DOIs	https://doi.org/10.1007/BF01983234
State	Published - May 1993
Externally published	Yes

Keywords

DNA delivery
Zea mays
electroporation electrolyte
heat shock

ASJC Scopus subject areas

Horticulture

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10.1007/BF01983234

Cite this

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title = "Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation",

abstract = "Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.",

keywords = "DNA delivery, Zea mays, electroporation electrolyte, heat shock",

author = "Songstad, {D. D.} and Halaka, {F. G.} and DeBoer, {D. L.} and Armstrong, {C. L.} and Hinchee, {M. A.W.} and Ford-Santino, {C. G.} and Brown, {S. M.} and Fromm, {M. E.} and Horsch, {R. B.}",

year = "1993",

month = may,

doi = "10.1007/BF01983234",

language = "English (US)",

volume = "33",

pages = "195--201",

journal = "Plant Cell, Tissue and Organ Culture",

issn = "0167-6857",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

AU - Songstad, D. D.

AU - Halaka, F. G.

AU - DeBoer, D. L.

AU - Armstrong, C. L.

AU - Hinchee, M. A.W.

AU - Ford-Santino, C. G.

AU - Brown, S. M.

AU - Fromm, M. E.

AU - Horsch, R. B.

PY - 1993/5

Y1 - 1993/5

N2 - Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.

AB - Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.

KW - DNA delivery

KW - Zea mays

KW - electroporation electrolyte

KW - heat shock

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Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

Abstract

Keywords

ASJC Scopus subject areas

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