TY - JOUR
T1 - Transient transformation of mammalian cells by MN protein, a tumor-associated cell adhesion molecule with carbonic anhydrase activity
AU - Zavada, Jan
AU - Zavadova, Zuzana
AU - Machon, Ondrej
AU - Kutinova, Luda
AU - Nemeckova, Sarka
AU - Opavsky, Rene
AU - Pastorek, Jaromir
PY - 1997
Y1 - 1997
N2 - The MN protein is associated with certain human carcinomas, but absent in most normal tissues. It is a transmembrane protein; its extracellular part contains a domain homologous with carbonic anhydrases (CAs) and a proteoglycan-like region. In the present study, we observed that cells (human CGL1 and mouse NIH3T3 cells) transfected with MN cDNA showed morphologic transformation, but reverted to normal phenotype after 4-5 weeks. This reversion was not due to the loss, silencing, or mutations of MN insert. We also found that MN protein exerted CA enzymatic activity, but this was not relevant for morphologic transformation of cells. MN is an adhesion protein, involved in cell-to-cell contacts, this probably could explain its role in tumorigenesis.
AB - The MN protein is associated with certain human carcinomas, but absent in most normal tissues. It is a transmembrane protein; its extracellular part contains a domain homologous with carbonic anhydrases (CAs) and a proteoglycan-like region. In the present study, we observed that cells (human CGL1 and mouse NIH3T3 cells) transfected with MN cDNA showed morphologic transformation, but reverted to normal phenotype after 4-5 weeks. This reversion was not due to the loss, silencing, or mutations of MN insert. We also found that MN protein exerted CA enzymatic activity, but this was not relevant for morphologic transformation of cells. MN is an adhesion protein, involved in cell-to-cell contacts, this probably could explain its role in tumorigenesis.
KW - Carbonic anhydrase
KW - Cell adhesion molecule
KW - Oncogene
KW - Tumor suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=0030898642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030898642&partnerID=8YFLogxK
U2 - 10.3892/ijo.10.4.857
DO - 10.3892/ijo.10.4.857
M3 - Article
C2 - 21533456
AN - SCOPUS:0030898642
SN - 1019-6439
VL - 10
SP - 857
EP - 863
JO - International journal of oncology
JF - International journal of oncology
IS - 4
ER -