TY - JOUR
T1 - Traumatic brain injury increases levels of miR-21 in extracellular vesicles
T2 - Implications for neuroinflammation
AU - Harrison, Emily B.
AU - Hochfelder, Colleen G.
AU - Lamberty, Benjamin G.
AU - Meays, Brittney M.
AU - Morsey, Brenda M.
AU - Kelso, Matthew L.
AU - Fox, Howard S.
AU - Yelamanchili, Sowmya V.
N1 - Publisher Copyright:
© 2016 The Authors.
PY - 2016/8
Y1 - 2016/8
N2 - Traumatic brain injury (TBI) is an important health concern and effective treatment strategies remain elusive. Understanding the complex multicellular response to TBI may provide new avenues for intervention. In the context of TBI, cell-cell communication is critical. One relatively unexplored form of cell-cell communication in TBI is extracellular vesicles (EVs). These membrane-bound vesicles can carry many different types of cargo between cells. Recently, miRNA in EVs have been shown to mediate neuroinflammation and neuronal injury. To explore the role of EV-associated miRNA in TBI, we isolated EVs from the brain of injured mice and controls, purified RNA from brain EVs, and performed miRNA sequencing. We found that the expression of miR-212 decreased, while miR-21, miR146, miR-7a, and miR-7b were significantly increased with injury, with miR-21 showing the largest change between conditions. The expression of miR-21 in the brain was primarily localized to neurons near the lesion site. Interestingly, adjacent to these miR-21-expressing neurons were activated microglia. The concurrent increase in miR-21 in EVs with the elevation of miR-21 in neurons, suggests that miR-21 is secreted from neurons as potential EV cargo. Thus, this study reveals a new potential mechanism of cell-cell communication not previously described in TBI.
AB - Traumatic brain injury (TBI) is an important health concern and effective treatment strategies remain elusive. Understanding the complex multicellular response to TBI may provide new avenues for intervention. In the context of TBI, cell-cell communication is critical. One relatively unexplored form of cell-cell communication in TBI is extracellular vesicles (EVs). These membrane-bound vesicles can carry many different types of cargo between cells. Recently, miRNA in EVs have been shown to mediate neuroinflammation and neuronal injury. To explore the role of EV-associated miRNA in TBI, we isolated EVs from the brain of injured mice and controls, purified RNA from brain EVs, and performed miRNA sequencing. We found that the expression of miR-212 decreased, while miR-21, miR146, miR-7a, and miR-7b were significantly increased with injury, with miR-21 showing the largest change between conditions. The expression of miR-21 in the brain was primarily localized to neurons near the lesion site. Interestingly, adjacent to these miR-21-expressing neurons were activated microglia. The concurrent increase in miR-21 in EVs with the elevation of miR-21 in neurons, suggests that miR-21 is secreted from neurons as potential EV cargo. Thus, this study reveals a new potential mechanism of cell-cell communication not previously described in TBI.
KW - Controlled cortical impact
KW - Exosomes
KW - MicroRNA
KW - Microglia
KW - Neuroinflammation
KW - Secondary injury
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UR - http://www.scopus.com/inward/citedby.url?scp=85081459741&partnerID=8YFLogxK
U2 - 10.1002/2211-5463.12092
DO - 10.1002/2211-5463.12092
M3 - Article
C2 - 27516962
AN - SCOPUS:85081459741
SN - 2211-5463
VL - 6
SP - 835
EP - 846
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 8
ER -