Global water shortage has driven the development of technologies in both water savings and water reuse. In this study, an innovative technological combination of biofilters packed with shredded tires and membrane bioreactors was investigated to reclaim greywater. Biofilm distribution was monitored. Membrane fouling mechanisms were studied in terms of extracellular polymeric substances and biomass accumulation on membranes with time, as well as identification of inorganic foulants at the molecular level with the aid of Raman spectroscopy. It was found that the biofilters removed up to 64.6 of 5-day biochemical oxygen demand, and hydraulic retention time had an impact on the performance of biofilters. The effluents from the membrane bioreactors met the guidelines for domestic wastewater reuse suggested by U.S. Environmental Protection Agency. The combination was thus proved to be feasible for greywater reclamation. The colorimetric method to measure extracellular polymeric substances indicated that protein might contribute to membrane fouling, and the Raman spectroscope detected gypsum (CaSO42H2O) on fouled membranes, which was likely to be the inorganic foulant.