Automatic brain segmentation has the potential to save time and resources for researchers and clinicians. We aimed to improve upon previously proposed methods by implementing the U-Net model and trialing various modifications to the training and inference strategies. The trials were performed and tested on the Multimodal Brain Tumor Segmentation dataset that provides MR images of brain tumors along with manual segmentations for hundreds of subjects. The U-Net models were trained on a training set of MR images from 369 subjects and then tested against a validation set of images from 125 subjects. The proposed modifications included predicting the labeled region contours, permutations of the input data via rotation and reflection, grouping labels together, as well as creating an ensemble of models. The ensemble of models provided the best results compared to any of the other methods, but the other modifications did not demonstrate improvement. Future work will look at reducing the level of the training augmentation so that the models are better able to generalize to the validation set. Overall, our open source deep learning framework allowed us to quickly implement and test multiple U-Net training modifications. The code for this project is available at https://github.com/ellisdg/3DUnetCNN.