Trichromatic concept optimizes MAD experiments in synchrotron X-ray crystallography

Takashi Kumasaka, Masaki Yamamoto, Eiki Yamashita, Hideaki Moriyama, Tatzuo Ueki

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The trichromatic concept is a new synchrotron beamline design that optimizes MAD experiments by reducing systematic experimental errors with three-colored and coaxial synchrotron X-ray beams produced by a tandem vertical undulator and trichromator. The concept enables rapid and flexible switching of three defined wavelengths, and extends the flexibility of experimental design for MAD data collection. Thus, we can collect MAD data taking into account time series effects such as radiation damage. The data based on the trichromatic concept gave a better quality electron density map than data collected by conventional methods. It was also revealed that multicolor diffraction using dichromatic or trichromatic X-ray beams is effective in rapid MAD data collection.

Original languageEnglish (US)
Pages (from-to)1205-1210
Number of pages6
JournalStructure
Volume10
Issue number9
DOIs
StatePublished - Sep 2002

Keywords

  • MAD
  • Synchrotron radiation
  • Trichromatic concept

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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