Triplication of one chromosome no. 15 with an altered C‐myc containing ecori fragment and elimination of the normal homologue in a T‐cell lymphoma line of akr origin (TIKAUT)

Zvi Wirschubsky, Francis Wiener, Jack Spira, Janos Sümegi, George Klein

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

An ouabain‐ and thioguanine‐resistant subline (TIKAUT) of spontaneous AKR lymphoma, TKA, was trisomic for chromosome 15 and contained a single 33 kb EcoRI fragment, containing the oncogene c‐myc. The original TKA lymphoma and derived in vitro line contained the same 33 kb fragment, as well as a normal 22 kb fragment. It has been concluded that the original 15‐trisomic TKA tumor has duplicated a 15‐chromosome that contained the changed fragment, while maintaining the normal fragment as well. Subsequently, in the derived TIKAUT line, the changed chromosome duplicated again, giving rise to three copies, and the normal homologue was eliminated altogether. This confirms our earlier somatic hybrid study showing that the duplicated 15‐chromosome of a T‐cell leukemia confers an advantage on the cell that favors tumorigenicity, whereas the normal homologue exerts a counteracting influence. Therefore, in the course of tumor progression, the changed chromosome tends to be amplified, whereas its normal homologue tends to be eliminated.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalInternational Journal of Cancer
Volume33
Issue number4
DOIs
StatePublished - Apr 15 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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