Abstract
Wheat streak mosaic virus (WSMV) is an eriophyid mite-transmitted virus of the genus Tritimovirus, family Potyviridae. Complete deletion of helper component-proteinase (HC-Pro) has no effect on WSMV virulence or disease synergism, suggesting that a different viral protein suppresses RNA silencing. RNA silencing suppression assays using Nicotiana benthamiana 16C plants expressing GFP were conducted with each WSMV protein; only P1 suppressed RNA silencing. Accumulation of GFP siRNAs was markedly reduced in leaves infiltrated with WSMV P1 at both 3 and 6 days post infiltration relative to WSMV HC-Pro and the empty vector control. On the other hand, helper component-proteinase (HC-Pro) of two species in the mite-transmitted genus Rymovirus, family Potyviridae was demonstrated to be a suppressor of RNA silencing. Symptom enhancement assays were conducted by inoculating Potato virus X (PVX) onto transgenic N. benthamiana. Symptoms produced by PVX were more severe on transgenic plants expressing WSMV P1 or potyvirus HC-Pro compared to transgenic plants expressing GFP or WSMV HC-Pro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 672-677 |
| Number of pages | 6 |
| Journal | Virus Research |
| Volume | 163 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2012 |
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Keywords
- Post-transcriptional gene silencing suppression
- Potyviridae
- Wheat streak mosaic virus
ASJC Scopus subject areas
- Cancer Research
- Virology
- Infectious Diseases