Tumor cell surface β1-6 branched oligosaccharides and lung metastasis

Yi Lu, Jill C. Pelling, William G. Chaney

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

NIH3T3 cells transfected with an activated Ha-ras oncogene were treated with L-PHA, the leukoagglutinin from red kidney beans. Cell lines resistant to L-PHA-mediated cytotoxicity were isolated and found to contain reduced levels of L-PHA-binding oligosaccharides. The levels of N-acetylglucosaminyltransferase V, the enzyme responsible for the initiation of the β1-6 branch, were reduced in L-PHA-resistant cells. Tumorigenicity in nude mice was unchanged by the change in oligosaccharide expression, but the ability to form lung tumors after intravenous injection was significantly reduced. These results demonstrate that the ability of NIH3T3 cells transfected with an activated Ha-ras oncogene to form lung tumors after intravenous injection into nude mice is reduced in all six L-PHA selected cell lines containing a reduction in β1-6 branched Asn-linked oligosaccharides.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalClinical & Experimental Metastasis
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1994

Keywords

  • GlcNAc-T V
  • lectin
  • lectin-affinity chromatography
  • metastasis
  • β1-6 branched oligosaccharides

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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