Background: The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). Methods: rhTNF (0.5 ng to 1.0 μg) was incorporated into the slow release polymers Hydron or HYPAN(®) and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN(®) released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. Conclusion: Under the circumstances tested, rhTNF is not angiogenic in vivo.
|Original language||English (US)|
|Number of pages||4|
|State||Published - May 1996|
- Rabbit cornea
- Tumor necrosis factor
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)