Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

Gregg D. Phillips; A. Marika Stone; Julie C. Schultz; Bryan D. Jones; Mark J. Lisowski; Margot L. Goodkin; David R. Knighton

doi:10.1002/(SICI)1097-0185(199605)245:1<53::AID-AR9>3.0.CO;2-G

Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

Gregg D. Phillips, A. Marika Stone, Julie C. Schultz, Bryan D. Jones, Mark J. Lisowski, Margot L. Goodkin, David R. Knighton

Anesthesiology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Background: The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). Methods: rhTNF (0.5 ng to 1.0 μg) was incorporated into the slow release polymers Hydron or HYPAN(®) and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN(®) released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. Conclusion: Under the circumstances tested, rhTNF is not angiogenic in vivo.

Original language	English (US)
Pages (from-to)	53-56
Number of pages	4
Journal	Anatomical Record
Volume	245
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1097-0185(199605)245:1<53::AID-AR9>3.0.CO;2-G
State	Published - May 1996

Keywords

Angiogenesis
Neovascularization
Rabbit cornea
Tumor necrosis factor

ASJC Scopus subject areas

Anatomy
Agricultural and Biological Sciences (miscellaneous)

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10.1002/(SICI)1097-0185(199605)245:1<53::AID-AR9>3.0.CO;2-G

Cite this

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title = "Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea",

abstract = "Background: The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). Methods: rhTNF (0.5 ng to 1.0 μg) was incorporated into the slow release polymers Hydron or HYPAN({\textregistered}) and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN({\textregistered}) released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. Conclusion: Under the circumstances tested, rhTNF is not angiogenic in vivo.",

keywords = "Angiogenesis, Neovascularization, Rabbit cornea, Tumor necrosis factor",

author = "Phillips, {Gregg D.} and Stone, {A. Marika} and Schultz, {Julie C.} and Jones, {Bryan D.} and Lisowski, {Mark J.} and Goodkin, {Margot L.} and Knighton, {David R.}",

year = "1996",

month = may,

doi = "10.1002/(SICI)1097-0185(199605)245:1<53::AID-AR9>3.0.CO;2-G",

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T1 - Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

AU - Phillips, Gregg D.

AU - Stone, A. Marika

AU - Schultz, Julie C.

AU - Jones, Bryan D.

AU - Lisowski, Mark J.

AU - Goodkin, Margot L.

AU - Knighton, David R.

PY - 1996/5

Y1 - 1996/5

N2 - Background: The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). Methods: rhTNF (0.5 ng to 1.0 μg) was incorporated into the slow release polymers Hydron or HYPAN(®) and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN(®) released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. Conclusion: Under the circumstances tested, rhTNF is not angiogenic in vivo.

AB - Background: The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). Methods: rhTNF (0.5 ng to 1.0 μg) was incorporated into the slow release polymers Hydron or HYPAN(®) and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN(®) released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. Conclusion: Under the circumstances tested, rhTNF is not angiogenic in vivo.

KW - Angiogenesis

KW - Neovascularization

KW - Rabbit cornea

KW - Tumor necrosis factor

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Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

Abstract

Keywords

ASJC Scopus subject areas

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