Tumor Progression in Four Mammary Epithelial Cell Lines Derived from the Same Patient

Vimla Band, Deborah Zajchowski, Karen Swisshelm, Douglas Trask, Victoria Kulesa, Ruth Sager, James Connolly, Craig Cohen

Research output: Contribution to journalArticlepeer-review

171 Scopus citations


Two primary and two metastatic cell lines with distinct phenotypes and genotypes have been established from a patient diagnosed as having infiltrating and intraductal mammary carcinoma (2IT series). AH four lines can be cultured in the same medium, DFCI-1, which also supports long-term growth of normal epithelial cells. Therefore, we have been able to compare normal and tumor cells at the cellular and molecular levels. The mammary origin of the 21T series was confirmed by using antibodies against the human milk fat globule antigen-2 epitope. The two primary tumor lines (2INT and 21PT) are both immortal and aneuploid, although only 2INT is tumorigenic in the nude mouse assay. The two populations derived from the metastatic pleural effusion (21MT-1 and 21MT-2) each exhibit distinct characteristics in morphology and growth factor requirements. The erbBl gene is amplified and overexpressed in all of these cell lines compared to normal epithelial cell controls. These four tumor cell lines from a single patient represent a mammary tumor progression series that has been established in long-term cell culture.

Original languageEnglish (US)
Pages (from-to)7351-7357
Number of pages7
JournalCancer Research
Issue number22
StatePublished - Nov 15 1990
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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