Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds

Yuguo Lei, Quinn K.T. Ng, Tatiana Segura

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The ability to genetically modify mesenchymal stem cells (MSCs) seeded inside synthetic hydrogel scaffolds would offer an alternative approach to guide MSC differentiation. In this report, we explored gene transfer to MSCs seeded on top or inside matrix metalloproteinase (MMP) degradable hydrogels that were loaded with DNA/poly(ethylene imine) (PEI) polyplexes. DNA/PEI polyplexes were encapsulated inside poly(ethylene glycol) (PEG) hydrogels crosslinked with MMP degradable peptides via Michael Addition chemistry. Gene transfer was visualized and quantified through using a vector encoding for green fluorescent protein and luciferase. We found that gene transfer to MSCs was possible for cells seeded both in two and three dimensions. The amount of luciferase expression was similar for cells seeded in two and three dimensions even though the number of cells in three dimensions is significantly higher, indicating that gene transfer to cells seeded in two dimensions is more efficient than for cells seeded in three dimensions. The use of hydrogel scaffolds that allow cellular infiltration to deliver DNA may result in long-lasting signals in vivo, which are essential for the regeneration of functional tissues.

Original languageEnglish (US)
Pages (from-to)910-917
Number of pages8
JournalMicroscopy Research and Technique
Volume73
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • Gene delivery
  • Hydrogel
  • Local delivery
  • Polyethylene glycol
  • Polyethylene imine

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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