Two-dimensional gels: An easy method for large quantities of proteins

Richard L. Barent; Thomas E. Elthon

doi:10.1007/BF02668910

Two-dimensional gels: An easy method for large quantities of proteins

Richard L. Barent, Thomas E. Elthon

Agronomy & Horticulture

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30 Scopus citations

Abstract

A new two-dimensional gel technique has been developed that makes it possible to load large amounts of protein and still retain good resolution. Protein samples are added to the isoelectric-focusing acrylamide solution prior to polymerization. Focusing times are short, making it possible to complete both dimensions of a two-dimensional gel in five to six hours. The technique yields larger amounts of separated protein for sequencing, and also greatly increases the quality of two-dimensional immunoblots.

Original language	English (US)
Pages (from-to)	338-344
Number of pages	7
Journal	Plant Molecular Biology Reporter
Volume	10
Issue number	4
DOIs	https://doi.org/10.1007/BF02668910
State	Published - Nov 1992

Keywords

Two-dimensional gels
protein sequencing

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02668910

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abstract = "A new two-dimensional gel technique has been developed that makes it possible to load large amounts of protein and still retain good resolution. Protein samples are added to the isoelectric-focusing acrylamide solution prior to polymerization. Focusing times are short, making it possible to complete both dimensions of a two-dimensional gel in five to six hours. The technique yields larger amounts of separated protein for sequencing, and also greatly increases the quality of two-dimensional immunoblots.",

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Two-dimensional gels: An easy method for large quantities of proteins

Abstract

Keywords

ASJC Scopus subject areas

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