Two-dimensional gels: An easy method for large quantities of proteins

Richard L. Barent, Thomas E. Elthon

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A new two-dimensional gel technique has been developed that makes it possible to load large amounts of protein and still retain good resolution. Protein samples are added to the isoelectric-focusing acrylamide solution prior to polymerization. Focusing times are short, making it possible to complete both dimensions of a two-dimensional gel in five to six hours. The technique yields larger amounts of separated protein for sequencing, and also greatly increases the quality of two-dimensional immunoblots.

Original languageEnglish (US)
Pages (from-to)338-344
Number of pages7
JournalPlant Molecular Biology Reporter
Volume10
Issue number4
DOIs
StatePublished - Nov 1 1992

Keywords

  • Two-dimensional gels
  • protein sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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