Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Susan C. Chapman, Aaron Lawson, William C. MacArthur, Russell J. Wiese, Robert H. Loechel, Maria Burgos-Trinidad, John K. Wakefield, Ram Ramabhadran, Teri Jo Mauch, Gary C. Schoenwolf

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

We report the first ubiquitous green fluorescent protein expression in chicks using a lentiviral vector approach, with eGFP under the control of the phosphoglycerol kinase promoter. Several demonstrations of germline transmission in chicks have been reported previously, using markers that produce tissue-specific, but not ubiquitous, expression. Using embryos sired by a heterozygous male, we demonstrate germline transmission in the embryonic tissue that expresses eGFP uniformly, and that can be used in tissue transplants and processed by in situ hybridization and immunocytochemistry. Transgenic tissue is identifiable by both fluorescence microscopy and immunolabeling, resulting in a permanent marker identifying transgenic cells following processing of the tissue. Stable integration of the transgene has allowed breeding of homozygous males and females that will be used to produce transgenic embryos in 100% of eggs laid upon reaching sexual maturity. These results demonstrate that a transgenic approach in the chick model system is viable and useful even though a relatively long generation time is required. The transgenic chick model will benefit studies on embryonic development, as well as providing the pharmaceutical industry with an economical bioreactor.

Original languageEnglish (US)
Pages (from-to)935-940
Number of pages6
JournalDevelopment
Volume132
Issue number5
DOIs
StatePublished - Mar 2005

Keywords

  • Chick
  • Development
  • GFP
  • Lentiviral
  • Transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Chapman, S. C., Lawson, A., MacArthur, W. C., Wiese, R. J., Loechel, R. H., Burgos-Trinidad, M., Wakefield, J. K., Ramabhadran, R., Mauch, T. J., & Schoenwolf, G. C. (2005). Ubiquitous GFP expression in transgenic chickens using a lentiviral vector. Development, 132(5), 935-940. https://doi.org/10.1242/dev.01652