Abstract
Background: UDP-glucose dehydrogenase (UGDH) mutants were engineered to perturb hexamer:dimer quaternary structure equilibrium. Results: Dimeric species of UGDH have reduced activity in vitro and in supporting hyaluronan production by cultured cells. Conclusion: Only dynamic UGDH hexamers support robust cellular function. Significance: Manipulation of UGDH activity by hexamer stabilization may offer new therapeutic options in cancer and other pathologies.
Original language | English (US) |
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Pages (from-to) | 35049-35057 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 49 |
DOIs | |
State | Published - Dec 6 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology